| 粘虫幼虫血淋巴中的凝集素 |
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| 引用本文： | 朱呈智,孙勇.粘虫幼虫血淋巴中的凝集素[J].昆虫学报,1992,35(4):399-404. |
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| 作者姓名： | 朱呈智 孙勇 |
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| 作者单位： | 南开大学生物系, 天津300071 |
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| 基金项目： | 高等学校博士学科点专项资助 |
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| 摘 要： | 粘虫Mythlmna separata Walker幼虫血淋巴中含有凝集某些脊椎动物红细胞的凝集素,凝集活性可被乳糖、岩藻糖或神经氨酸抑制.用CNBr-sepharose 4B 进行亲和层析从血淋巴中分离的凝集素成分比较复杂,聚丙烯酰胺凝胶电泳显示三条区带,SDS聚丙烯酰胺凝胶电泳出现6个亚基,亚基分子量分别为71000、65000、56000、35000、33000及31000道尔顿.
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| 关 键 词： | 粘虫幼虫 凝集素 |
THE LECTIN IN THE HEMOLYMPH OF MYTHIMNA SEPARATA LARVAE |
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| ZHU CHENG-ZHI SUN YONG CHENG ZHEN-HENG.THE LECTIN IN THE HEMOLYMPH OF MYTHIMNA SEPARATA LARVAE[J].Acta Entomologica Sinica,1992,35(4):399-404. |
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| Authors: | ZHU CHENG-ZHI SUN YONG CHENG ZHEN-HENG |
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| Institution: | Department of Biology, Nankai Uviversity, Tianjin 300071 |
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| Abstract: | Hemolymph of Mythimna separata larvae was found to be able to agglutinate erythrocytes of some vertebrates. Hemaglutinating activity was specifically inhibited by fucose. lactose and neuraminic acid. A lectin was purified from hemolymph of the larvae by affinity chroma-tography on CNBr-Sepharose 4B column. The purified lectin showed three protein bands on polyacrylamide gel electrophoresis and six subunits on SDS-polyacrylamide gel electrophoresis. The molecular weights of these subunits were 71,000, 65,000, 56,000, 35,000, 33,000 and 31,000 daltons, respectively. |
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| Keywords: | Mythimna separata larvae-lectin |
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