人工注射Dnmt3 siRNA对意大利蜜蜂雌蜂发育的影响

为了研究人工注射DNA甲基化转移酶3 (DNA methyltransferase3, Dnmt3) siRNA对意大利蜜蜂Apis mellifera ligustica雌蜂的Dnmt3酶活性、 Dnmt3 mRNA表达量、 dynactin p62基因甲基化水平以及蜜蜂形态指标之间的影响和关系, 分别给3组3日龄的意大利蜜蜂幼虫人工注射50 ng Ringer, uth siRNA和Dnmt3 siRNA溶液。在幼虫的发育过程中, 测定每组幼虫头部的Dnmt3酶活性、 Dnmt3 mRNA表达量、 dynactin p62基因甲基化水平以及刚羽化雌性蜜蜂的初生重、 体长、 前翅长、 前翅宽、 吻长、 第3背板长等形态指标。结果表明: 人工注射Dnmt3 siRNA不仅可以显著降低意大利蜜蜂幼虫头部Dnmt3酶活性、 Dnmt3 mRNA表达量和dynactin p62基因整体甲基化水平, 同时显著提高了刚羽化雌性蜜蜂的初生重、 体长、 第3背板长。结果说明Dnmt3的改变可以影响雌性意蜂幼虫的发育。

Influence of injecting Dnmt3 siRNA on the development of females of the Italian honeybee.Apis mellifera ligustica

 In order to investigate the effects of injecting Dnmt3 siRNA on Dnmt3 activity, Dnmt3 mRNA expression level, methylation status of the dynactin p62 gene and the morphological characters in female Apis mellifera ligustica, 50 ng Ringer, uth siRNA and Dnmt3 siRNA were injected into 3 d old female larvae of A. mellifera ligustica reared in an incubator, respectively. Dnmt3 activity, Dnmt3 mRNA expression level, and dynactin p62 methylation level in larval head and the morphological characters including body weight, body length, forewing length, forewing width, proboscis length and 3rd tergum length of newly emerged female adults were examined. The results showed that Dnmt 3 activity, the Dnmt3 expression level and the methylation level of dynactin p62 in larval head were significantly inhibited after the larvae were injected with Dnmt3 siRNA, while the body weight, body length and 3rd tergum length of newly emerged female adults increased, indicating that the changes of Dnmt3 have an effect on female larvae of A. mellifera ligustica during their development.