不同保藏处理的昆虫标本DNA提取及其随机扩增多态DNA反应

实验利用CTAB法对柳二十斑叶甲Chrysomela vigintipunctata (Scopoli)、异色瓢虫Harmonia axyridis Pollas、七星瓢虫Coccinella septempunctata Linnaeus、小地老虎Agrotis ypsilon (Rottemberg)、红蜻Crocothemis servilia Drury、无齿稻蝗Oxya abentata Willemse和中华稻蝗Oxya chinensis (Thunberg)等7种昆虫进行了基因组DNA提取。从自然干燥标本、烘干标本及酒精浸泡标本获得的DNA均可用于RAPD-PCR反应，且烘干标本、酒精浸泡标本提取效果优于自然干燥标本。这种提取方法简便易行,容易掌握，且耗资小于其它分子生物学方法。

DNA extraction and RAPD-PCR of insect specimens preserved with different meth-ods

Genomic DNA were extracted and amplified using the polymerase chain reaction and arbitrary primers from specimens of seven insect species: Chrysomela vigintipunctata (Scopoli) (Coleptera: Chrycomelidae), Harmonia axyridis Pollas and Coccinella septempunctata Linnaeus (Coleoptera: Coccinellidae), Agrotis ypsilon (Rottemberg) (Lepidoptera: Noctuidae), Crocothemis servilia Drury (Odonata: Libellulidae), and Oxya abentata Willemse and O. chinensis (Thunberg) (Orthoptera: Catantopidae). Of the specimens assayed, some had been naturally dried, some oven-dried and the remainder had been preserved in alcohol. The results suggest that, with respect to DNA extraction, oven-dried specimens and those preserved in alchohol are better than naturally dried specimens. Extraction of DNA extraction using the CTAB method was easier, and more economical than other methods.