白背飞虱单克隆抗体的制备及其特性的研究

应用杂交瘤技术，制备出4株高度特异性的白背飞虱单克隆抗体，分别命名为WPH-1H9、WPH-2B6、WPH-2E12和WPH-3F12。这些抗体与其它8种昆虫未发生交叉反应，其中WPH-2B6可与白背飞虱所有虫态发生反应，其余3株只与卵和雌成虫发生反应。应用免疫双扩散法鉴定抗体类型及亚类，结果表明：WPH-2B6为IgG_2b亚类，其余均为IgG₁亚类。SDS-聚丙烯酰胺凝胶电泳和Western blot印迹分析表明，白背飞虱抗原主要由分子量分别为182、116、66.2及40 kD的4个多肽组成，其中WPH-2B6与182、116 kD的多肽结合，其余3株的单抗只与116 kD的多肽具有亲和性。最后对这些单克隆抗体在捕食作用研究中的应用潜力进行了讨论。

Development and characterization of monoclonal antibodies to the white-backed planthopper Sogatella furcifera

Four species and stage specific monoclonal antibodies (McAbs) to whole homogenates of adults of the white backed planthopper, Sogatella furcifera , were developed using hybridoma technology. These McAbs did not cross react with any other insect species tested with an indirect enzyme linked immunosorbent assay (ELISA).Moreover, of the four McAbs, the McAb, namely WPH 2B6, was highly specific to all the stages of white backed planthopper while the other three reacted only with both the egg and adult female antigens. Immunodiffusion showed that the McAb WPH 2B6 was IgG 2b subclass while all the others belonged to IgG 1 subclasses using standard goat anti mouse antibodies specific to each mouse antibody subclass (Sigma Chemical Co. Ltd., Pode. Dorset). SDS PAGE and Western blot analyses indicated that the adult antigen was mainly composed of four polypeptides with molecular weights estimated at 182 kD, 116 kD, 66 2 kD and 40 kD, respectively, and McAb WPH 2B6 reacted with two polypeptides with molecular weights of 116 kD and 182 kD respectively while the other three were bound specifically to one with molecular weight of 116 kD. Finally, the potential application of these McAbs to study the predation of natural enemies on the white backed planthopper is discussed.