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转基因杨树对杨小舟蛾体内三种保护酶活力的影响
引用本文:郭同斌,嵇保中,蒋继宏,杜伟,诸葛强,黄敏仁.转基因杨树对杨小舟蛾体内三种保护酶活力的影响[J].昆虫学报,2006,49(3):381-386.
作者姓名:郭同斌  嵇保中  蒋继宏  杜伟  诸葛强  黄敏仁
作者单位:徐州市森林病虫防治检疫站
基金项目:国家研究与开发专项项目(JY03-B-25),江苏省高新技术资助项目(BG2001313,BG2003306)
摘    要:用转Bt单基因(FB56)和转BtCpTI双价基因(D18)杨树叶片饲喂杨小舟蛾Micromelalopha troglodyta (Graeser),采用酶活力测定方法研究了转基因杨树对幼虫中肠和血淋巴三种保护酶(SOD、CAT 和POD)活力的影响。结果表明,幼虫中肠SOD活力显著升高,而CAT和POD活力受到了显著抑制。用FB56处理24 h,杨小舟蛾幼虫中肠SOD活力增加了41.7%,与对照有显著差异;而用D18处理,SOD活力增加了25.3%,与对照差异不显著。用FB56处理24 h,中肠CAT活力受到显著抑制,36 h后抑制能力不强;而用D18处理,CAT活力全程受到显著抑制。用FB56和D18处理36 h,中肠POD活力均受到显著抑制,分别比对照下降70.1%和898%。转基因杨树通过扰乱幼虫中肠SOD、CAT和POD三种保护酶的动态平衡,使虫体内自由基清除遇到了障碍,从而对其产生了毒害作用。转双价基因杨树对幼虫中肠保护酶的毒害作用大于转单基因杨树。用2种转基因杨树叶片饲喂杨小舟蛾幼虫后其血淋巴SOD和POD活力均没有受到显著影响,但CAT活力均显著高于对照,转基因杨树对血淋巴保护酶系统的影响小于对中肠保护酶系统的影响。

关 键 词:转基因杨树  杨小舟蛾  保护酶  SOD  CAT  POD  
文章编号:0454-6296(2006)03-0381-06
收稿时间:08 17 2005 12:00AM
修稿时间:11 25 2005 12:00AM

Effect of transgenic poplars on the activities of three protective enzymes in Micromelalopha troglodyta (Graeser) (Lepidoptera: Notodontidae)
GUO Tong-Bin,JI Bao-Zhong,JIANG Ji-Hong,DU Wei,ZHUGE Qiang,HUANG Min-Ren.Effect of transgenic poplars on the activities of three protective enzymes in Micromelalopha troglodyta (Graeser) (Lepidoptera: Notodontidae)[J].Acta Entomologica Sinica,2006,49(3):381-386.
Authors:GUO Tong-Bin  JI Bao-Zhong  JIANG Ji-Hong  DU Wei  ZHUGE Qiang  HUANG Min-Ren
Institution:Forest Pest Management and Quarantine Station of Xuzhou City
Abstract:Effect of transgenic poplars on the activities of three protective enzymes (SOD, CAT and POD) in the midgut and haemolymph of Micromelalopha troglodyta (Graeser) larvae fed with leaves of transgenic poplars expressing Bt gene only (FB56) and expressing Bt and CpTI genes (D18) was studied in the laboratory using enzyme activities determination. The results showed that the SOD activity in the midgut of the larvae was raised significantly, while the CAT and POD activities were controlled significantly. When the larvae were fed with the leaves of FB56 for 24 hours, the activity of SOD in the midgut of the larvae was raised by 41.7%, which had significant differences with CK. But SOD activity was raised by 25.3% after treating with D18, which had no significant difference with CK. After 24 hours feeding on FB56, the activity of CAT in the midgut of the larvae was restrained significantly, but the restraining ability was not strong after 36 hours. But CAT activity was controlled significantly after treating with D18. FB56 and D18 all restrained the activity of POD in the midgut of the larvae greatly after 36 hours treatment, and the activity decreased by 70.1% and 89.8% respectively. Thus, the transgenic poplars could delay the cleaning of free radicals in the body of M. troglodyta, and disturb the dynamic balance of SOD, CAT and POD in the midgut of the larvae, which was the one of acting mechanisms. The harm of the transgenic poplars expressing both Bt and CpTI to the larvae is stronger than transgenic poplars expressing Bt alone. The activities of SOD and POD in the haemolymph of the larvae had no significant difference with CK after treating with leaves of two kinds of transgenic poplars, but the activity of CAT had a significant increase compared with CK. Thus, the influence of transgenic poplars on the endogenous protective enzymes in the haemolymph was smaller than that in the midgut.
Keywords:Transgenic poplars  Micromelalopha troglodyta  protective enzymes  SOD  CAT  POD
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