保幼激素类似物对斜纹夜蛾核型多角体病毒在宿主血淋巴中增殖动态的影响

通过研究保幼激素类似物(juvenile hormone analogues, JHA) methoprene对斜纹夜蛾核型多角体病毒（Spodoptera litura nucleopolyhedrovirus, SpltNPV）在宿主血淋巴中增殖的影响，以探明JHA促进 SpltNPV增殖的初步机制，为阐明JHA促进病毒增殖提供更全面的理论依据。应用SDS PAGE及Western blot法，分析了methoprene对SpltNPV多角体蛋白(polyhedron, POLH)在宿主斜纹夜蛾6龄幼虫血淋巴中合成的影响。结果表明：经methoprene处理后2～3天可明显促进幼虫血淋巴液中POLH的合成。在此基础上，通过荧光定量PCR检测，发现methoprene对SpltNPV在幼虫血淋巴液复制的影响主要发生在处理后的第4和第5天，该期间polh基因的拷贝数比对照显著增加，拷贝数的峰值达1.22×10¹⁰/mL。

Effect of the juvenile hormone analogue methoprene on multiplication of Spodoptera litura nucleopolyhedrovirus in the host hemolymph

Juvenile hormone analogues (JHA) can promote multiplication of Spodoptera litura nucleopolyhedrovirus (SpltNPV). The effect of methoprene on synthesis of polyhedrin in the hemolymph of the 6th instar larvae of the host S. litura was studied by using SDS-PAGE and immunoblot methods. The results showed that the synthesis of polyhedrin in the host hemolymph was promoted in 2－3 days post treatment. The effect of methoprene on replication of SpltNPV-polh in hemolymph of the 6th instar larvae was investigated by real-time quantitative PCR. The results showed that replication of SpltNPV-polh was promoted significantly from the 4th day to the 5th day post methoprene treatment, during which polh copies increased to 1.22×10¹⁰ copy/mL.