Biosynthesis and biotechnological application of non-canonical amino acids: Complex and unclear |
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Authors: | Huibin Zou Lei Li Tongtong Zhang Mengxun Shi Nan Zhang Jingling Huang Mo Xian |
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Institution: | 1. College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;2. CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China |
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Abstract: | Compared with the better-studied canonical amino acids, the distribution, metabolism and functions of natural non-canonical amino acids remain relatively obscure. Natural non-canonical amino acids have been mainly discovered in plants as secondary metabolites that perform diversified physiological functions. Due to their specific characteristics, a broader range of natural and artificial non-canonical amino acids have recently been applied in the development of functional materials and pharmaceutical products. With the rapid development of advanced methods in biotechnology, non-canonical amino acids can be incorporated into peptides, proteins and enzymes to improve the function and performance relative to their natural counterparts. Therefore, biotechnological application of non-canonical amino acids in artificial bio-macromolecules follows the central goal of synthetic biology to: create novel life forms and functions. However, many of the non-canonical amino acids are synthesized via chemo- or semi-synthetic methods, and few non-canonical amino acids can be synthesized using natural in vivo pathways. Therefore, further research is needed to clarify the metabolic pathways and key enzymes of the non-canonical amino acids. This will lead to the discovery of more candidate non-canonical amino acids, especially for those that are derived from microorganisms and are naturally bio-compatible with chassis strains for in vivo biosynthesis. In this review, we summarize representative natural and artificial non-canonical amino acids, their known information regarding associated metabolic pathways, their characteristics and their practical applications. Moreover, this review summarizes current barriers in developing in vivo pathways for the synthesis of non-canonical amino acids, as well as other considerations, future trends and potential applications of non-canonical amino acids in advanced biotechnology. |
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Keywords: | Non-canonical amino acids Biosynthesis Synthetic biology ncAA non-canonical amino acid β-ODAP α-ODAP DJK Djenkolic acid HCA Homocysteic acid L-Trp L-tryptophan 5-HTP 5-HT 5-hydroxytryptamine MSG monosodium glutamate NOS nitric oxide synthase HCY Homocysteine GDH glutamate dehydrogenase Aib 2-aminoisobutyric acid PEG polyethylene glycol pAcF PHGDH Phosphoglycerate dehydrogenase azo-Phe azobenzene phenylalanine cAA canonical amino acid PrK 6 |
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