| The Post-meiotic Deficicent Anther1 （PDA1） gene is required for post-meiotic anther development in rice |
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| 作者姓名： | Lifang Hu Hexin Tan Wanqi Liang Dabing Zhang |
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| 作者单位： | [1]School of Life Science and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China [2]Bio-X Research Center,Key Laboratory of Genetics ＆ Development and Neuropsychiatric Diseases,Ministry of Education,Shanghai Jiao Tong University,Shanghai 200240,China |
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| 基金项目： | china，上海市学科建设项目 |
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| 摘 要： | To understand the molecular mechanism of male reproductive development in the model crop rice,we isolated a complete male sterile mutant post-meiotic deficient anther1 (pda1) from a γ-ray-treated rice mutant library.Genetic analysis revealed that the pda1 mutant was controlled by a recessive nucleus gene.The pda1 mutant anther seemed smaller with white appearance.Histological analysis demonstrated that the pda1 mutant anther undergoes normal early tapetum development without obvious altered meiosis.However,the pda1 mutant displayed obvious defects in postmeiotic tapetal development,abnormal degeneration occurred in the tapetal cells at stage 9 of anther development.Also we observed abnormal lipidic Ubisch bodies from the tapetal layer of the pda1 mutant,causing no obvious pollen exine formation.RT-PCR analysis indicated that the expression of genes involved in anther development including GAMYB,OsC4 and Wax-deficient anther1 (WDA1) was greatly reduced in the pda1 mutant anther.Using map-based cloning approach,the PDA1 gene was finely mapped between two markers HLF610 and HLF627 on chromosome 6 using 3,883 individuals of F2 population.The physical distance between HLF610 and HLF627 was about 194 kb.This work suggests that PDA1 is required for post-meiotic tapetal development and pollen/microspore formation in rice.
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| 关 键 词： | 减数分裂 基因表达 花药 水稻 绒毡层细胞 邮政 突变体 雄性不育 |
| 收稿时间： | 24 September 2009 |
The Post-meiotic Deficicent Anther1(PDA1)gene is required for post-meiotic anther development in rice |
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| Lifang Hu,Hexin Tan,Wanqi Liang,Dabing Zhang.The Post-meiotic Deficicent Anther1(PDA1)gene is required for post-meiotic anther development in rice[J].Journal of Genetics and Genomics,2010,37(1):37-46. |
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| Authors: | Lifang Hu Hexin Tan Wanqi Liang Dabing Zhang |
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| Institution: | 1. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;2. Bio-X Research Center, Key Laboratory of Genetics & Development and Neuropsychiatric Diseases, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200240, China;1. Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing University of Agriculture, Beijing, 102206, China;2. Lab of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310029, China;1. Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University (SJTU)–University of Adelaide Joint Centre for Agriculture and Health, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China;2. Department of Oriental Medicinal Biotechnology and Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Youngin, 446-701, South Korea;3. School of Agriculture, Food, and Wine, University of Adelaide, South Australia 5064, Australia;1. Peking University Institute of Advanced Agricultural Sciences, Weifang 261325, Shandong, China;2. Shenzhen Institute of Molecular Crop Design, Shenzhen 518107, China;3. College of Life Sciences, Capital Normal University, Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing 100048, China;1. Programa de Pós-graduação em Genética e Biologia Molecular, Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, 91509-900, RS, Brazil;2. Programa de Pós-graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, 91509-900, RS, Brazil;3. Programa de Pós-graduação em Botânica, Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, 91509-900, RS, Brazil;4. Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Av. da República, 2780-157, Oeiras, Portugal |
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| Abstract: | To understand the molecular mechanism of male reproductive development in the model crop rice,we isolated a complete male sterile mutant post-meiotic deficient anther1(pda1)from a γ-ray-treated rice mutant library.Genetic analysis revealed that the pda1 mutant was controlled by a recessive nucleus gene.The pda1 mutant anther seemed smaller with white appearance.Histological analysis demonstrated that the pda1 mutant anther undergoes normal early tapetum development without obvious altered meiosis.However,the pda1 mutant displayed obvious defects in postmeiotic tapetal development,abnormal degeneration occurred in the tapetal cells at stage 9 of anther development.Also we observed abnormal lipidic Ubisch bodies from the tapetal layer of the pda1 mutant,causing no obvious pollen exine formation.RT-PCR analysis indicated that the expression of genes involved in anther development including GAMYB,OsC4 and Wax-deficient antherl(WDA1)was greatly reduced in the pda1 mutant anther.Using map-based cloning approach,the PDA1 gene was finely mapped between two markers HLF610 and HLF627 on chromosome 6 using 3,883 individuals of F2 population.The physical distance between HLF610 and HLF627 was about 194 kb.This work suggests that PDA1 is required for post-meiotic tapetal development and pollen/microspore formation in rice. |
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| Keywords: | rice anther degeneration pollen exine mapping |
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