Prediction of the Reaction Equilibrium of Biocatalytic Reactions in Aqueous-Organic Two-Phase Systems |
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| Authors: | Anja E. M. Janssen Albert Van Der Padt Klaas Van't Riet |
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| Affiliation: | a Department of Food Science, Food and Bioprocess Engineering Group, Wageningen Agricultural University, Wageningen, EV, The Netherlands |
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| Abstract: | Biocatalytic reactions can be carried out in aqueous-organic two-phase systems. Several models to describe the thermodynamically-determined equilibrium position in such systems have appeared in the literature. Some of these models are only valid for dilute systems, whereas others can also be used for nondilute systems. In this paper, these models are described and compared. It is explained in what way the equilibrium constants of each model can be used to predict the product concentration in different organic solvents. |
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| Keywords: | biocatalysis enzymatic synthesis equilibrium organic solvent choice two-phase system |
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