DNase I sensitivity of Microtus agrestis active,inactive and reactivated X chromosomes in mouse-Microtus cell hybrids |
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Authors: | Bat-Sheva Kerem Veronika Kottusch-Geiseler Vera Kalscheuer Ruth Goitein Karl Sperling Menashe Marcus |
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Institution: | (1) Department of Genetics, Hebrew University, Jerusalem, Israel;(2) Institut für Humangenetik, Freie Universität Berlin, Heubnerweg 6, D-1000 Berlin 19 |
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Abstract: | We isolated Microtus agrestis-mouse somatic cell hybrid clones which had retained either the active or the inactive M. agrestis X chromosome. In both hybrid clones the X chromosomes retained their original chromatin conformation as studied by the in situ nick translation technique — the active X chromosome retained its high sensitivity to DNase I while the inactive one remained insensitive. A clone in which the hypoxanthine guanine phosphoribosyltransferase (HPRT) gene had been spontaneously reactivated was isolated from the hybrid containing the inactive X chromosome. The in situ nick translation technique was used to study possible DNA conformation changes in the euchromatin of the inactive X chromosome with special reference to the reactivated HPRT locus. We found that the euchromatin in this X chromosome exhibited the same low sensitivity to DNase I as is characteristic of the inactive X chromosome.Professor Marcus passed away on 2 January 1987 |
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