| 杏鲍菇抗烟草花叶病毒蛋白的筛选 |
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| 引用本文： | 付鸣佳,林健清,吴祖健,林奇英,谢联辉.杏鲍菇抗烟草花叶病毒蛋白的筛选[J].微生物学报,2003,43(1):29-34. |
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| 作者姓名： | 付鸣佳 林健清 吴祖健 林奇英 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所,福州,350002 |
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| 基金项目： | 福建省科技厅重点项目 ( 99-Z1- 93) |
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| 摘 要： | 采用离子交换层析和凝胶层析方法，从杏鲍菇干样中分离得到多个蛋白组分，经枯斑寄主检测，发现多个蛋白组分都有抗烟草花叶病毒(TMV)的活性，对TMV的抑制率均在70%以上，高者可达99%。其中xb68Ab已得到了纯化，分子量约为23.7kD，在心叶烟和苋色藜上它对TMV侵染的抑制率分别达到99.43%和98.9%。
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| 关 键 词： | 杏鲍菇， 抗病毒蛋白， 烟草花叶病毒蛋白 |
| 文章编号： | 0001-6209(2003)01-0029-06 |
Screening of Proteins Anti-Tobacco Mosaic Virus in Pleurotus eryngii |
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| Fu Mingjia,Lin Jianqing,Wu Zujian,Lin Qiying,Xie Lianhui =.Screening of Proteins Anti-Tobacco Mosaic Virus in Pleurotus eryngii[J].Acta Microbiologica Sinica,2003,43(1):29-34. |
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| Authors: | Fu Mingjia Lin Jianqing Wu Zujian Lin Qiying Xie Lianhui = |
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| Institution: | Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. |
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| Abstract: | Proteins were obtained by ion |exchange chromatography and gel filtration from the dry sample of %Pleurotus eryngii. % In terms of detection on local lesion host, these components were confirmed to be anti |TMV and their inhibition rates reach more than 70%, or even 99%. The protein named xb68Ab of MW 23 ^7kD was purified by ion |exchange chromatography and gel filtration. its inhibition rate against TMV are 99 ^43% and 98 ^9% on %Nicotiana glutionosa % and %Chenopodium amaranticolor %, respectively. |
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| Keywords: | Pleurotus eryngii Antiviral protein Tobacco mosaic virus |
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