| 青霉菌立体选择性环氧化顺丙烯磷酸产生磷霉素 |
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| 引用本文： | 石家骥,崔福绵,葛猛.青霉菌立体选择性环氧化顺丙烯磷酸产生磷霉素[J].微生物学报,2001,41(3):353-356. |
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| 作者姓名： | 石家骥 崔福绵 葛猛 |
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| 作者单位： | 中国科学院微生物研究所 |
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| 基金项目： | 中国科学院重大研究课题资助(KY951-A1-304-01-02),微生物资源前期开发国家重点实验室客座课题 |
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| 摘 要： | 由土壤中分离出一株青霉 (Penicilliumsp .) ,编号F5,能选择性的将顺丙烯磷酸环氧化为磷霉素 ,在pH7 5、2 8℃、2 80r min条件下培养 6d ,底物浓度 0 3%时 ,产物浓度达 2 2mg mL ,产率 41 % ;底物浓度 0 6%时产率 8%。转化产物经磷霉素敏感菌生物检测 ,TLC检测 ,并与标准品比较 ,确证为磷霉素。
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| 关 键 词： | 青霉 顺丙烯磷酸 磷霉素 生物转化 |
| 文章编号： | 0001-6209(2001)03-0353-04 |
THE EPOXIDATION OF cis-PROPENYLPHOPHONIC ACID TO FOSFOMYCIN BY PENCILLIUM SP. |
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| Shi Jiaji Cui Fumian Ge meng.THE EPOXIDATION OF cis-PROPENYLPHOPHONIC ACID TO FOSFOMYCIN BY PENCILLIUM SP.[J].Acta Microbiologica Sinica,2001,41(3):353-356. |
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| Authors: | Shi Jiaji Cui Fumian Ge meng |
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| Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. |
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| Abstract: | One strain of Penicillium sp. F5, found from soil sample, was able to stereo-specific epoxidation of cis-propenylphosphonic acid(PPOH) to fosfomycin(-)-(1R, 2S)-1, 2-epoxypropylphosphonic acid, FOM] during the cultivation. The product (FOM) was identified by thin layer chromatography and microbiological assay. Under the culture conditions of 28 degrees C pH7.5 and 280 r/min, 0.3% concentration of PPOH, reached a level of 2.2 mg/ml of FoM and the yield was 41%. When the PPOH concentration was 0.6%, the yield was 8%. |
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| Keywords: | Penicillium sp Cis\|propenylphophonic acid Fosfomycin Biotransformation |
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