Thermal stability of the Z-conformation of the tetranucleoside triphosphate (m5dC-dG)2 |
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Authors: | D Genest B Hartmann N T Thuong M Ptak M Leng |
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Institution: | 1. Centre de Biophysique Moléculaire, C.N.R.S., 45045 Orleans Cedex, France;2. Université d''Orléans, 45045 Orleans Cedex, France |
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Abstract: | The tetranucleoside triphosphate d(m5C-G)2 has been studied in solution by circular dichroism and 31P nuclear magnetic resonance as a function of temperature, in presence of 3 M NaClO4. It is shown that in such high ionic strength d(m5C-G)2 may adopt a Z-like conformation for temperatures lower than 5 degrees C. At these temperatures, another conformation, in slow equilibrium with the Z-like one, is also detected. Increasing the temperature leads to a transition from the Z-like conformation to intermediate forms before melting. It is demonstrated that these intermediates are not the B form. |
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