Vegetative multiplication of sugarbeet through in vitro culture of inflorescence pieces |
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| Authors: | M F Coumans-Gilles Cl Kevers M Coumans E Ceulemans Th Gaspar |
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| Institution: | (1) Hormonologie Fondamentale et Appliquée, Institut de Botanique (B22), Université de Liège - Sart Tilman, B-4000 Liège, Belgium |
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| Abstract: | In vitro vegetative multiplication of sugarbeet was obtained by culturing of inflorescence explants. Subapical segments or
5-mm-long tips from nine varieties developed axillary shoots (up to 50 per tip) on a medium containing indolebutyric acid
(IBA) and benzylaminopurine (BAP). Zeatin was ineffective as cytokinin. Gibberellic acid (GA3) enhanced the process. Such vegetative shoots were subsequently isolated and were each allowed to develop up to 20 supplementary
axillary shoots on a multiplication medium containing IBA, BAP, and naphthaleneacetic acid (NAA). Rooting of shoots was obtained
in the absence of growth regulators and plants were established. |
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| Keywords: | vegetative multiplication sugarbeet in vitro culture organogenesis Beta vulgaris |
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