Three-dimensional reconstruction of a human metaphase chromosome from electron micrographs |
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| Authors: | George Harauz Lisa Borland Gunter F Bahr Elmar Zeitler Marin van Heel |
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| Institution: | (1) Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, D-1000 Berlin 33;(2) Department of Cellular Pathology, Armed Forces Institute of Pathology, 20306 Washington, DC, USA |
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| Abstract: | A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from –60 to +60 degrees. The reconstructed structure is about 3.0  m long, 1.6 m wide, and 0.8 m thick. The mass distribution was fairly homogeneous within the chromatids and neither a hollow nor a dense core was observed. The distribution and course of fibers observed are most consistent with a looping model of chromosome structure. |
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