Thidiazuron-induced <Emphasis Type="Italic">in vitro</Emphasis> shoot organogenesis of the medicinal plant <Emphasis Type="Italic">Arnebia euchroma</Emphasis> (Royle) Johnst |
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Authors: | Bo?Jiang Email author" target="_blank">Ying-Gen?YangEmail author Yi-Ming?Guo Zhong-Chen?Guo Yong-Zhe?Chen |
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Institution: | (1) Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China;(2) School of Life Science, Shandong University, 250100 Jinan, China |
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Abstract: | Summary An efficient in vitro propagation system was developed for Arnebia euchroma, an important Chinese traditional medicinal plant. The present study utilized thidiazuron (TDZ) for the induction of shoot
organogenesis on cotyledon and hypocotyl explants. The maximal number of shoots was obtained on the modified Linsmaier and
Skoog (LS) medium supplemented with 1.0 mgl−1 (4.5 μM) TDZ for 12d on cotyledon explants (8.6 shoots per cotyledon explant). Other cytokinins (kinetin and 6-benzyladenine) and
auxin (α-naphthaleneacetic acid) were not efficient in inducing regeneration on cotyledon explants. Browning of the basal
portion of the subcultured shoots could be significantly reduced when they were cultured on the modified LS medium supplemented
with 100 mgl−1 (33.3 μM) polyvinylpyrrolidone. Well-developed shoots formed roots on the same medium containing 1.0 mgl−1 (4.9 μM) indole-3-butyric acid. The efficient regeneration protocol reported here provides an important means of micropropagation
of this plant. Furthermore, this protocol is essential to future genetic improvement of plants via transformation protocols. |
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Keywords: | Arnebia euchroma medicinal plant polyvinylpyrrolidone shoot organogenesis thidiazuron |
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