Long-term multiplication of the Chinese hamster ovary (CHO) cell line in a serum-free medium |
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Authors: | Francois Gasser Philippe Mulsant Michel Gillois |
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Institution: | (1) Laboratoire de Génétique Cellulaire, Centre de Recherches INRA de Toulouse, B.P. 12, 31320 Castanet-Tolosan, France |
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Abstract: | Summary A new synthetic medium (referred to as GC3) that supports the growth of the Chinese hamster ovary cell line has been developed. It is composed of a 1∶1 mixture of Ham's
F12 and modified Eagle's minimum essential (MEM.S) mediums supplemented with transferrin (10 μg/ml), insulin (80 mU/ml), and
selenium (1×10−7
M). Other more simple supplementations of our basal medium MEM.S/F12 (transferrin+insulin, transferrin+selenium, ferrous iron+selenium)
also give good cell growth responses. Fibronectin or serum pretreatment is not needed for cellular attachment and spreading.
Our culture system is characterized by a continuous serum-free cultivation (more than 200 doublings), a clonal growth, a high
density proliferation, and a rapid growth rate near that of cells in serum-supplemented medium. |
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Keywords: | serum-free medium Chinese hamster ovary cells long-term culture insulin transferrin ferrous iron |
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