RNA Control of HIV-1 Particle Size Polydispersity |
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| Authors: | Cendrine Faivre-Moskalenko Julien Bernaud Audrey Thomas Kevin Tartour Yvonne Beck Maksym Iazykov John Danial Morgane Lourdin Delphine Muriaux Martin Castelnovo |
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| Affiliation: | 1. Université de Lyon, Laboratoire de Physique, Ecole Normale Supérieure de Lyon, CNRS, Lyon, France.; 2. CNRS UMR 5236, Centre d''études d''agents Pathogènes et Biotechnologies pour la Santé, Montpellier, France.; 3. Université de Lyon, INSERM, U758, Ecole Normale Supérieure de Lyon, Lyon, France.; Institut National de la Santé et de la Recherche Médicale, France, |
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| Abstract: | ![]()
HIV-1, an enveloped RNA virus, produces viral particles that are known to be much more heterogeneous in size than is typical of non-enveloped viruses. We present here a novel strategy to study HIV-1 Viral Like Particles (VLP) assembly by measuring the size distribution of these purified VLPs and subsequent viral cores thanks to Atomic Force Microscopy imaging and statistical analysis. This strategy allowed us to identify whether the presence of viral RNA acts as a modulator for VLPs and cores size heterogeneity in a large population of particles. These results are analyzed in the light of a recently proposed statistical physics model for the self-assembly process. In particular, our results reveal that the modulation of size distribution by the presence of viral RNA is qualitatively reproduced, suggesting therefore an entropic origin for the modulation of RNA uptake by the nascent VLP. |
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