Isolation and physiological study of an amylolytic strain of Lactobacillus plantarum |
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Authors: | Eric Giraud Alain Brauman Simon Keleke Bertrand Lelong Maurice Raimbault |
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Institution: | (1) Laboratoire de Biotechnologie, Centre Orstom, 2051 Avenue du val de Montferrand, B. P. 5045, F-34032 Montpellier Cedex 1, France;(2) Laboratoire de microbiologie, Centre Orstom, B. P. 181, Brazzaville, Congo |
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Abstract: | Summary An amylolytic lactic acid bacterium identified as Lactobacillus plantarum was isolated from cassava roots (Manihot esculenta var. Ngansa) during reting. The amylolytic enzyme synthesized was an extracellular -amylase with an optimum pH of 5.0 and an optimum temperature of 55° C. Cultured on starch, the strain displayed a growth rate of 0.43 h–1, a biomass yield of 0.19 g·g–1 and a lactate yield of 0.81 g·g–1. The growth kinetics were similar on starch and glucose. Sufficient enzyme was synthesized and starch hydrolysis was not a limiting factor for growth. Biosynthesis of the enzyme was observed when the glucose concentration was less than 6.7 g·l–1 and reached up to 4 IU·ml–1 at the end of the fermentation.
Offprint requests to: M. Raimbault |
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