Production of Neisseria gonorrhoeae pili (fimbriae) in Pseudomonas aeruginosa. |
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Authors: | P A Hoyne R Haas T F Meyer J K Davies and T C Elleman |
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Institution: | Division of Biomolecular Engineering, Commonwealth Scientific and Industrial Research Organisation, Parkville, Victoria, Australia. |
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Abstract: | Pseudomonas aeruginosa K/2PfS, when transformed with an expression plasmid harboring the pilin gene (pilE1) of Neisseria gonorrhoeae MS11, was able to express and assemble gonococcal pilin monomers into surface-associated pili, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and immunoelectron microscopy. Concomitant with the expression of gonococcal pili in P. aeruginosa was the virtual loss of production of P. aeruginosa K/2PfS pili normally associated with the host cell. |
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