| 鸭圆环病毒全基因组克隆与序列分析 |
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| 引用本文： | 傅光华,程龙飞,施少华,彭春香,陈红梅,黄瑜.鸭圆环病毒全基因组克隆与序列分析[J].病毒学报,2008,24(2):138-143. |
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| 作者姓名： | 傅光华 程龙飞 施少华 彭春香 陈红梅 黄瑜 |
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| 作者单位： | 福建省农业科学院,畜牧兽医研究所,福州,福建,350013;福建省农业科学院,畜牧兽医研究所,福州,福建,350013;福建省农业科学院,畜牧兽医研究所,福州,福建,350013;福建省农业科学院,畜牧兽医研究所,福州,福建,350013;福建省农业科学院,畜牧兽医研究所,福州,福建,350013;福建省农业科学院,畜牧兽医研究所,福州,福建,350013 |
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| 基金项目： | 国家自然科学基金，福建省财政专项-福建省农业科学院科技创新团队建设基金 |
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| 摘 要： | 为研究鸭圆环病毒全基因组的分子生物学特性,运用重叠PCR技术从鸭组织脏器提取的DNA中扩增出2条核苷酸序列,拼接后对其核酸组成、基因组结构及病毒的遗传变异进行分析.结果表明所获病毒核酸为大小1 995nt的环型DNA,包含6个ORF,与登录在GenBank中克隆株MuDCV(AY228555)的同源性高达97.4%,可见所扩增的核酸序列为鸭圆环病毒基因组序列.
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| 关 键 词： | 鸭圆环病毒 全基因组克隆 序列分析 |
| 文章编号： | 1000-8721(2008)02-0138-06 |
| 修稿时间： | 2007年3月19日 |
Genome Cloning and Sequence Analysis of Duck Circovirus |
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| FU Guang-hua,CHENG Long-fei,SHI Shao-hua,PENG Chun-xiang,CHEN Hong-mei,HUANG Yu.Genome Cloning and Sequence Analysis of Duck Circovirus[J].Chinese Journal of Virology,2008,24(2):138-143. |
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| Authors: | FU Guang-hua CHENG Long-fei SHI Shao-hua PENG Chun-xiang CHEN Hong-mei HUANG Yu |
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| Institution: | Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China. |
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| Abstract: | To reveal the molecular biological characteristics of genome of circovirus in infected ducks, two nucleotide fragments were amplified by overlapping PCRs using DNA extracted from various tissues of ducks. After they had been assembled together, the nucleotide components, the genome organization and the phylogenetic scale of the sequence were analyzed. The results showed that the obtained sequence is a circular DNA with a total length of 1995nt. It contains 6 open reading frames (ORFs), and shares a high identity of 97.4% with the MuDCV circovirus sequence presented in GenBank (AY228555). These results indicate that the amplified product stems from duck circovirus sequence. |
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| Keywords: | duck circovirus genome cloning sequence analysis |
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