Metabolically engineered oilseed crops with enhanced seed tocopherol |
| |
Authors: | Karunanandaa Balasulojini Qi Qungang Hao Ming Baszis Susan R Jensen Pamela K Wong Yun-Hua H Jiang Jian Venkatramesh Mylavarapu Gruys Kenneth J Moshiri Farhad Post-Beittenmiller Dusty Weiss James D Valentin Henry E |
| |
Institution: | Monsanto Company, 800 N. Lindbergh Boulevard, St. Louis, MO 63167, USA. |
| |
Abstract: | Tocochromanols (tocopherols and tocotrienols) are important lipid soluble antioxidants and are an essential part of the mammalian diet. Oilseeds are particularly rich in tocochromanols with an average concentration 10-fold higher than other plant tissues. Here we describe a systematic approach to identify rate-limiting reactions in the tocochromanol biosynthetic pathway, and the application of this knowledge to engineer tocochromanol biosynthesis in oilseed crops. Seed-specific expression of genes encoding limiting tocochromanol pathway enzymes in soybean increased total tocochromanols up to 15-fold from 320 ng/mg in WT seed to 4800 ng/mg in seed from the best performing event. Although WT soybean seed contain only traces of tocotrienols, these transgenic soybean accumulated up to 94% of their tocochromanols as tocotrienols. Upon crossing transgenic high tocochromanol soybean with transgenic high alpha-tocopherol soybean, the vitamin E activity in the best performing F2-seed was calculated to be 11-fold higher than the average WT soybean seed vitamin E activity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|