The effect of trans-10, cis-12 conjugated linoleic acid on gene expression profiles related to lipid metabolism in human intestinal-like Caco-2 cells |
| |
| Authors: | Eileen F. Murphy Guido J. Hooiveld Michael Müller Raffaelle A. Calogero Kevin D. Cashman |
| |
| Affiliation: | 1Department of Food and Nutritional Sciences, University College Cork, Cork, Ireland ;2Department of Medicine, University College Cork, Cork, Ireland ;3Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, PO Box 8129, 6700EV Wageningen, The Netherlands ;4Bioinformatics and Genomics Unit, Department of Clinical and Biological Sciences, University of Turin, Orbassano, Italy |
| |
| Abstract: | We conducted an in-depth investigation of the effects of conjugated linoleic acid (CLA) on the expression of key metabolic genes and genes of known importance in intestinal lipid metabolism using the Caco-2 cell model. Cells were treated with 80 μmol/L of linoleic acid (control), trans-10, cis-12 CLA or cis-9, trans-11 CLA. RNA was isolated from the cells, labelled and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis were preformed using Bioconductor. Gene ontology analysis (GO) revealed a significant enrichment (P < 0.0001) for the GO term lipid metabolism with genes up-regulated by trans-10, cis-12 CLA. Trans-10, cis-12 CLA, but not cis-9, trans-11 CLA, altered the expression of a number of genes involved in lipid transport, fatty acid metabolism, lipolysis, β-oxidation, steroid metabolism, cholesterol biosynthesis, membrane lipid metabolism, gluconeogenesis and the citrate cycle. These observations warrant further investigation to understand their potential role in the metabolic syndrome. |
| |
| Keywords: | Conjugated linoleic acid Gene expression Caco-2 cells |
| 本文献已被 SpringerLink 等数据库收录！ |
|
