Somatic embryogenesis from styles of lemon (Citrus limon) |
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Authors: | Francesco Carimi Fabio De Pasquale Francesco Giulio Crescimanno |
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Institution: | (1) Centro di Studio per il Miglioramento Genetico degli Agrumi, Consiglio Nazionale delle Ricerche c/o Facoltà di Agraria, Viale delle Scienze, I-90128 Palermo, Italy |
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Abstract: | Lemon Citrus limon (L.) Burm.] styles were treated with different growth regulators for induction of somatic embryos. Styles and stigmas were dissected from flowers and cultured on a Murashige and Skoog (MS) basal medium supplemented with 4.52 M 2,4-dichlorophenoxyacetic acid and 13.3 M 6-benzyladenine. Callus was induced from the style base 2 weeks after the treatment initiation, and embryos appeared 2 months later.Abbreviations BA
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- MS
Murashige & Skoog (1962)
- NAA
-naphthaleneacetic acid |
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Keywords: | plant regeneration tissue culture |
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