抗白粉病小麦染色体组型的分子标记与生化标记分析

应用与小麦第六同源群有关的分子和生化标记，包括ＤＮＡ探针ｐＳｃ５·３Ｈ３和ｐＳＲ１６７以及同工酶Ｅｓｔ－５和ａ－Ａｍｙ－１，对来自六倍体小黑麦Ｂｅａｇｌｅ与普通小麦科冬５８杂交后代Ｆ１花粉植株的抗白粉病株系Ｍ２４．Ｍ０９及Ｍ１７进行了分析。结果表明，Ｍ２４、Ｍ０９及Ｍ１７不同程度地含有黑麦染色体成分，而且电泳谱带差别较大，据此推断，Ｍ０９为６ＲＬ的易位系。因此，生化和分子标记不仅可以用于确定外源片段的存在，而且可以帮助确定染色体组型和外源片段的位置

Biochemical and Molecular Marker Analysis of the Chromosome Composition of Powdery mildew -resistant Wheat

Biochemical and molecular markers involved in the homoeologous group 6, including Est-5, a-Amy-1, pSc5 . 3H3 and pSR167, were used to analyze the anther culture-derived lines M24, M09 and M17 in respect of chromosome composition pattern. These lines were resistant to powdery mildew and derived from F1 of common wheat Kedong 58 crossed with hexaploid Triticale Beagle. The results showed that these three lines contained rye chromosome segments to deferent extents and had distinctive banding types upon electmphoresis. From these data M09 was deduced to be a translocation line. These markers could be used not only to prove the existance of alien chromosome segments in wheat background, but also to reveal the chromosome composition pattern to some extent.