可诱导慢病毒载体的优化策略及应用

以1型人免疫缺陷病毒(HIV-1)为基础构建的慢病毒载体具有可感染非分裂细胞、免疫反应小、携带的基因片段容量大和可整合进宿主基因组而长期表达等优点,因而成为最理想的基因转移载体之一。可诱导慢病毒载体介导的可诱导基因表达系统能够有效控制目的基因表达,扩大了慢病毒载体的临床应用潜能,成为很有前景的基因治疗载体。主要介绍带有四环素和其他几种诱导系统的可调控性慢病毒载体及其改进,以及可诱导慢病毒载体在RNA干扰中的应用。

Application and Optimizational Strategy for Inducible Lentiviral Vectors

As human immunological virus type-1 (HIV-1) based lentiviral vector holds the characteristics of transfection to non-dividing cells, low rate of immunological response, larger capacity of transfer gene fragments and integrating into host genome for long-term expression of therapeutic gene, therefore it becomes one of the idealest gene transfer vectors in gene therapy. Transgene expression could be efficiently controlled by using the inducible lentivectors incorporating regulatable system,which expands the potential of lentivectors for a wide array of clinical gene transfer application, therefore it becomes a potential viral vector in gene therapy. Improvements of tetracycline and other kinds of induc-ible lentivector, and its application in gene therapy for RNA interference are emphasized in this review.