毕赤酵母的非甲醇诱导系统研究进展

毕赤酵母是一种能以甲醇为唯一碳源生长的甲基营养型酵母,常用作表达外源蛋白的细胞工厂。目前毕赤酵母常用的高效启动子AOX1(PAOX1)是一个严格的底物依赖型启动子,受甲醇的严格诱导,受葡萄糖、甘油、乙醇等非甲醇碳源抑制。但是甲醇有毒、易燃、易爆的特性使得其在食用、医用产品生产等领域受到很大的限制。本文将主要从PAOX1机制改造、新型启动子和非甲醇诱导物研发的角度阐述毕赤酵母非甲醇诱导的研究进展。

Advances in non-methanol induction system of Komagataella phaffii for heterologous protein production

Komagataella phaffii (K. phaffii) is a methyltrophic yeast that can grow with methanol as the sole carbon source to express heterologous proteins. At present, the efficient promoter AOX1 (P AOX1 ), commonly used in K. phaffii , is a strict substrate-dependent promoter, which is strictly induced by methanol and inhibited by non-methanol carbon sources such as glucose, glycerin, ethanol and so on. However, the toxic, flammable and explosive characteristics of methanol restrict the production of edible, medical products and other fields. In this paper, the research progress of non-methanol induction in K. phaffii was introduced from the point of view of P AOX1 mechanism modification, new promoter and non-methanol inducer development.