| 婴幼儿粪便中双歧杆菌的分离及其菌株特性研究 |
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| 引用本文： | 赵笑笑,王光强,夏永军,张汇,艾连中,熊智强.婴幼儿粪便中双歧杆菌的分离及其菌株特性研究[J].工业微生物,2019,49(2):1-6. |
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| 作者姓名： | 赵笑笑 王光强 夏永军 张汇 艾连中 熊智强 |
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| 作者单位： | 上海理工大学医疗器械与食品学院,上海食品微生物工程技术研究中心,上海200093;上海理工大学医疗器械与食品学院,上海食品微生物工程技术研究中心,上海200093;上海理工大学医疗器械与食品学院,上海食品微生物工程技术研究中心,上海200093;上海理工大学医疗器械与食品学院,上海食品微生物工程技术研究中心,上海200093;上海理工大学医疗器械与食品学院,上海食品微生物工程技术研究中心,上海200093;上海理工大学医疗器械与食品学院,上海食品微生物工程技术研究中心,上海200093 |
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| 基金项目： | 国家重点研发计划;国家自然科学基金;上海市自然科学基金 |
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| 摘 要： | 为筛选具有潜在益生功能的双歧杆菌,本研究采用改良MRS培养基,从婴幼儿粪便中分离到5株菌株:AR499,AR667,AR668,AR669和AR610。通过16S r DNA测序分别鉴定为两歧双歧杆菌,短双歧杆菌,动物双歧杆菌,长双歧杆菌和假小链双歧杆菌。对其耐酸耐胆盐能力和黏附性能进行测试,结果表明,菌株AR499耐酸性较好,菌株AR610有较强的耐胆盐能力,菌株AR499的自动聚集能力和细胞表面疏水性都较高。实验表明,菌株AR499可作为一株耐受性和黏附性能较好的益生菌进一步深入研究,以期应用于优良双歧杆菌产品的开发。
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| 关 键 词： | 双歧杆菌 分离 耐酸耐胆盐 黏附 |
Screening and characteristics of bifidobacteria from infant feces |
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| ZHAO Xiaoxiao,WANG Guangqiang,XIA Yongjun,ZHANG Hui,AI Lianzhong,XIONG Zhiqiang.Screening and characteristics of bifidobacteria from infant feces[J].Industrial Microbiology,2019,49(2):1-6. |
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| Authors: | ZHAO Xiaoxiao WANG Guangqiang XIA Yongjun ZHANG Hui AI Lianzhong XIONG Zhiqiang |
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| Institution: | (Shanghai Engineering Research Center of Food Microbiology, School ofMedical Instrument andFood Engineering, University of Shanghai for Science and Technology,Shanghai 200093) |
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| Abstract: | Bifidobacteria with potential probiotic properties were isolated from infant feces by modified MRS medium. Five different strains AR499, AR667, AR668, AR669 and AR610 were identified as Bifdobacterium bifdum, B. longum, B. breve, B. pseudocatenulatum and B. animalis by 16S rDNA sequencing. The adhesion properties and the tolerance to acid and bile salt were determined in these 5 strains. The results showed that B. bifdum AR499 had stronger acid resistance, automatic aggregation ability and cell surface hydrophobicity, and B. pseudocatenulatum AR610 had stronger bile salt resistance. The experiments demonstrated that B. bifdum AR499 could be used as a potential probiotic with high stress tolerance and good adhesion ability for further research, in order to be applied to the development of excellent bifidobacteria products. |
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| Keywords: | bifidobacterium isolation acid and bile salt tolerance adhesion |
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