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Biochemical and autoradiographic studies of TRH receptors in sections of rabbit spinal cord |
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| Authors: | N A Sharif N S Pilotte D R Burt |
| Affiliation: | Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA |
| Abstract: | Receptors for thyrotropin-releasing hormone (pGlu-His-Pro-NH2, TRH) on thaw-mounted sections of rabbit spinal cord have been identified biochemically and visualized by light microscopic autoradiography. Binding of [3H] [3-Me-His2]TRH to 20 microns sections exhibited high apparent affinity and a pharmacological specificity almost identical to that previously demonstrated for spinal TRH receptors in membranes. In autoradiograms, the highest density of TRH receptors appeared in the substantia gelatinosa of the dorsal gray and around the central canal, with intermediate levels in the ventral gray. |
| Keywords: | TRH thyrotropin-releasing hormone SC spinal cord MeTRH i.p. intraperitoneally DMSO dimethylsulfoxide equilibrium dissociation constant density of binding sites cpm counts per min concentration inhibiting by 50% |
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