The effects of the buffer hepes on the division potential of WI-38 cells |
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| Authors: | H. R. Massie H. V. Samis M. B. Baird |
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| Affiliation: | (1) Masonic Medical Research Laboratory, Bleecker Street, 13501 Utica, New York |
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| Abstract: | Summary N-2-Hydroxyethylpiperazine-N′-2-ethane sulfonic acid (HEPES) and N-tris(hydroxymethyl)methyl-2-aminoethane sulfonic acid (TES) have been found to be nontoxic substitutes for bicarbonate buffer in cell culture media. WI-38 embryonic human lung cells have been carried beyond the 60th passage with both HEPES and TES buffers. |
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