| 用gFM31探针进行谷子品种的指纹分析 |
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| 引用本文： | 王志民,刘春吉.用gFM31探针进行谷子品种的指纹分析[J].遗传学报,1996,23(3):228-233. |
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| 作者姓名： | 王志民 刘春吉 |
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| 作者单位： | 河北省农林科学院谷子研究所 |
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| 摘 要： | 用高度多态性探针ｇＦＭ３１对５９个谷子（ＳｅｔａｒｉａｉｔａｒｌｉｃａＢｅａｕｖ．）品种（包括地方品种、育成品种及品系）进行ＤＮＡ指纹分析，共鉴别出５８种类型，而黑谷和黑粒１５１６给出完全相同的带型，二者有可能是同一材料。通过对ｇＦＭ３１ＤＮＡ序列分析，未发现其中有小卫星ＤＮＡ和微卫星ＤＮＡ序列。该探针虽在谷子品种中显示很高的多态性，但与禾谷类的其他物种的ＤＮＡ杂交信号微弱，表现出较强的谷子基因组特异性。
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| 关 键 词： | 谷子RFLP，品种鉴别，指纹分析 |
Fingerprinting Varieties of Foxtail Millet Using Probe gFM31 |
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| Wang Zhimin, Liu Chunji,Wang Runqi,M.D.Gale.Fingerprinting Varieties of Foxtail Millet Using Probe gFM31[J].Journal of Genetics and Genomics,1996,23(3):228-233. |
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| Authors: | Wang Zhimin Liu Chunji Wang Runqi MDGale |
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| Abstract: | Fifty-nine genotypes(landraces,varieties or breeder's lines)of foxtail millet(Setaria italica Beauv.)were used in DNA fingerprinting with a highly polymorphic probe, xPSF 31. Fifty-eight types were distingushed,with only two genotypes giving identical band patterns.DNA sequencing of xPSF31 did not reveal any relationship with mini-or micro-satellite sequences.The probe revealed only weak signals in genomic DNA from other cereal species,wheat,barley,maize,rice,and pearl millet,indicating that xPSF31 is a Setaria genus specific sequence. |
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| Keywords: | Setaria italica RFLP Cultivar Identification Fingerprinting |
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