Characterization of Promoter Activities of Four Different Japanese Flounder Promoters in Transgenic Zebrafish |
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Authors: | Ryosuke Yazawa Ikuo Hirono Takashi Aoki |
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Institution: | (1) Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato Tokyo, 108-8477, Japan |
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Abstract: | An important consideration in transgenic research is the choice of promoter for regulating the expression of a foreign gene.
In this study several tissue-specific and inducible promoters derived from Japanese flounder Paralichthys olivaceus were identified, and their promoter activity was examined in transgenic zebrafish. The 5′ flanking regions of the Japanese
flounder complement component C3, gelatinase B, keratin, and tumor necrosis factor (TNF) genes were linked to green fluorescence
protein (GFP) as a reporter gene. The promoter regulatory constructs were introduced into fertilized zebrafish eggs. As a
result we obtained several stable transgenic zebrafish that displayed green fluorescence in different tissues. Complement
component C3 promoter regulated GFP expression in liver, and gelatinase B promoter regulated it in the pectoral fin and gills.
Keratin promoter regulated GFP expression in skin and liver. TNF gene promoter regulated GFP expression in the pharynx and
heart. TNF promoter had lipoplysaccharide-inducible activity, such that when transgenic embryos were immersed lipopolysaccharide,
GFP expression increased in the epithelial tissues. These 4 promoters regulated the expression of GFP in different patterns
in transgenic zebrafish. |
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Keywords: | Japanese flounder tissue-specific promoter inducible promoter green fluorescence protein (GFP) transgenic zebrafish |
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