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Small interfering RNA targeting CD81 ameliorated arthritis in rats |
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| Authors: | Shuji Nakagawa Hiroki Mori Toshikazu Kubo |
| Affiliation: | a Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan b Molecular Biology and Clinical Diagnosis, Shujitsu University School of Pharmacy, 1-6-1 Nishigawara, Okayama 703-8516, Japan c Department of Oral Pathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8525, Japan |
| Abstract: | CD81 belongs to a family of cell-surface protein (tetraspanin) known as one of the up-regulated elements in rheumatoid arthritis synoviocytes. In this study, the therapeutic effect of small interfering RNA targeting CD81 (siCD81) was examined by in vivo electroporation method. Treatment with siCD81 significantly ameliorated paw swelling of collagen-induced arthritic (CIA) rats. In histological examination, hypertrophy of synovium, bone erosion, and degeneration of articular cartilage were milder in rats treated with siCD81 than in the control group and the non-specific siRNA group. Expression of synoviolin, a rheumatoid regulator, was suppressed by siCD81. Thus, therapeutic intervention by targeting CD81 may be used in the treatment of rheumatoid arthritis. |
| Keywords: | Arthritis siRNA CD81 Synoviolin Collagen-induced arthritic rat Synovial tissue Intraarticular injection In vivo electroporation |
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