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| 姜花属种间杂交胚拯救研究 | |
| 引用本文： | 熊友华,,庄雪影,刘念.姜花属种间杂交胚拯救研究[J].广西植物,2011,31(4):541-544. |
| 作者姓名： | 熊友华 庄雪影 刘念 |
| 作者单位： | 1. 仲恺农业工程学院园艺园林学院,广州510225;华南农业大学林学院,广州510642 2. 华南农业大学林学院,广州,510642 3. 仲恺农业工程学院园艺园林学院,广州,510225 |
| 基金项目： | 广东省科技计划项目(2010B020305013)~~ |
| 摘 要： | 为提高姜花属种间杂交胚挽救中幼胚萌发率,以白姜花×金姜花的胚珠为试材,研究不同胚珠发育时期、不同培养基及低温处理果实对幼胚萌发率的影响.结果表明,白姜花×金姜花胚挽救的适宜培养基是MS+0.1 mg/L BA十0.1 mg/L NAA；接种时期以60 d的幼胚培养效果最佳；低温处理果实3～6 d能有效提高幼胚的萌发率. |
| 关 键 词： | 姜花属 种间杂交 胚拯救 |
Study on embryo rescue of interspecific hybrids between Hedychium |
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| XIONG You-Hua,ZHUANG Xue-Ying,LIU Nian.Study on embryo rescue of interspecific hybrids between Hedychium[J].Guihaia,2011,31(4):541-544. | |
| Authors: | XIONG You-Hua ZHUANG Xue-Ying LIU Nian |
| Institution: | XIONG You-Hua1,2,ZHUANG Xue-Ying2,LIU Nian1(1.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China,2.College of Forestry,South China Agricultural University,Guangzhou 510642,China) |
| Abstract: | The object of this study was to improve the germination capacity of embryoes of Hedychium hybrids through embryo rescue in vitro.Hybrid ovules of H.coronarium×H.gardnerianum were used to investigate the effect of different growth stage of embryo,different culture medium and low-temperature treatment on the germination capacity of embryoes.The results indicated that MS medium supplemented with 0.1 mg/L BA and 0.1 mg/L NAA showed suitable for embryo rescue in vitro culture.It was showed that the optimum germination capacity of embryoes reached 40.6%,which was obtained by taking 60 day old immature embryo as explant.Low-temperature treatment of fruits for 3-6 d,had a positive effect on improvement of the germination capacity of embryoes. |
| Keywords: | Hedychium interspecific hybridization embryo rescue |
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