Respiration-dependent proton translocation and the mechanism of protonmotive force generation in Nitrobacter winogradskyi |
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| Authors: | Heinz-Georg Wetzstein Stuart J Ferguson |
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| Institution: | Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham, B15 2TT, U.K.;Institut für Mikrobiologie der Universität Göttingen, D-3400 Göttingen, F.R.G. |
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| Abstract: | Abstract Proton translocation associated with electron flow to oxygen has been observed with cells of Nitrobacter winogradskyi in the presence of either potassium ferrocyanide or isoascorbate plus N , N , N ', N ' tetramethyl- p -phenylenediamine. The data are consistent with a proton pumping function for the terminal oxidase, cytochrome aa 3, in this organism as the mechanism for generating a protonmotive force. The failure of previous work with Nitrobacter 4] to detect proton translocation linked to oxidation of nitrite, the physiological substrate, is discussed. |
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| Keywords: | Cytochrome oxidase (aa3) proton pumping chemiosmosis nitrite oxidation |
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