Effects of L-Tryptophan on Indoleamines and Catecholamines in Discrete Brain Regions of Wild Type and Lurcher Mutant Mice |
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Authors: | Reader Tomás A Le Marec Nathalie Ase Ariel R Lalonde Robert |
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Institution: | (1) Centre de Recherche en Sciences Neurologiques, Département de physiologie, Faculté de médecine, Université de Montréal, Montréal, Québec, Canada;(2) Unité de Neurologie du Comportement, Neurobiologie et Neuropsychologie, CHUM/Campus Hôtel-Dieu de Montréal, Montréal, Québec, Canada;(3) Département de physiologie, Faculté de médecine, Université de Montréal, Montréal, Québec, H3C 3J7, Canada |
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Abstract: | The biochemical parameters of the serotoninergic system were examined in wild type mice and Lurcher mutants after chronic treatment (40 days) with the serotonin (5-HT) precursor L-tryptophan (50 mg/kg; i.p.). Tissue contents in 5-HT, dopamine and noradrenaline, as well as some of their metabolites, were measured in frontal cortex, neostriatum, thalamus, brainstem, cerebellum and spinal cord by high-performance liquid chromatography. The tissue levels were used as a biochemical index of the function of the monoamine innervations in this animal model of cerebellar ataxia. The results show that Lurcher mutants retain higher concentrations of L-tryptophan and total indoleamines, but that 5-HT is probably stored in a non-releasable compartment. In the particular case of the hypoplastic cerebellum, the reorganization of 5-HT nerve terminals leads to an accrued indoleamine synthesis, indicating that the Lurcher mutants can accumulate 5-HT, but do not utilize it efficiently in synaptic transmission. |
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Keywords: | Serotonin 5-hydroxyindole-3-acetic acid 5-hydroxytryptophol dopamine noradrenaline cerebellar ataxia HPLC |
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