Thidiazuron promotes adventitious shoot regeneration from pothos (Epipremnum aureum) leaf and petiole explants |
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Authors: | Luping Qu Jianjn Chen Richard J Henny Yingfeng Huang Russell D Caldwell Cynthia A Robinson |
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Institution: | (1) IFAS. Mid-Florida Research and Education Center, University of Florida, 2725 Binion Road, 32703 Apopka, FL |
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Abstract: | Summary Regeneration of adventitious shoots of pothos (Epipremnum aureum Linden and Andre) ‘Jade’ was obtained using leaf and petiole explants preprated from shoot tips of 3-yr-old greenhouse-grown
plants. Explants were cultured on Murashige and Skoog (MS) basal medium supplemented with thidiazuron (TDZ), 6-(4-hydroxy-3-methy-trans-2-butenyl-amino)purine
(zeatin) or N-isopentenylaminopurine (2iP) individually with α-naphthaleneacetic acid (NAA) in 18 combinations. Callus was initiated from
cut surface and along the midrib or major vein of leaf sections. Shoot regeneration from leaf and petoole explants occurred
in 30d on medium containing 1, 5 or 10μM TDZ with 0.5 or 1.0μM NAA except petioles on medium with 10 μM TDZ and 1.0 μM NAA where regeneration failed. More time (50d) was needed for shoot regeneration when explants were cultured on medium containing
either 2iP or zeatin with NAA. Regeneration frequencies were up to 20% and 50% for leaf and petiole explants, respectively.
Shoot numbers per responding explant attained 30 for leaf and petiole explants on medium containing TDZ but only one to four
on medium containing either 2iP or zeatin. These results indicate that TDZ is a more effective cytokinin for in vitro regeneration of pothos than either zeatin or 2iP.. Shoots elongated readily and rooted well on MS basal medium, without plant
growth regulators. Plantlets acclimatized rapidly and grew vigorously in the greenhouse after transfer to pots containing
a commerecial potting medium. |
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Keywords: | growth regulators ornamental foliage plants thidiazuron tissue culture |
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