Staurosporine引起正常和肿瘤细胞内Ca~(2 )和CaM水平发生变化

低剂量Staurosporine可以使正常细胞可逆地阻断于G1期,但对肿瘤细胞的周期运行不发生任何影响。本文利用显微光度术,测定了单个细胞内Ca~(2 )、活化钙调素和总钙调素的含量,结果表明:5ng/mL staurosporine作用于细胞18h,使正常细胞2BS G1和S期总CaM含量降低;而BGC-823细胞各周期时相总钙调素含量不发生改变;钙活化钙调素增加。Staurosporine阻断2BS细胞于G1期而不影响BGC-823的周期运行可能与Stauro-Sporine使2BS G1期细胞的钙调素降低以及抑制了2BS细胞的P~(107)磷酸化有关。

CHANGES OF INTRACELLULAR CAICIUM, CALMODULIN IN NORMAL AND TUMOR CELLS TRAGGERED BY STAUROSPORINE

Treated with low dosage staurosporine, the normal cell 2 BS were arrested in G 1 phase, but tumor cell BGC-823 were not. We measured the intracellular calcium, calmodulin, Ca2 -activited calmodulin (Ca2 -CaM) contents of single cells according to cell cycle with micro photometry. Our results showed that treatment of 5 ng/ ml staurosporine for 18 h caused the changes of calcium and calmodulin. In 2 BS cells, CaM leval decreased in G 1 and S phase. In each cell cycle phase of BGC-823 cells, there was no changes in CaM level, but Ca2 -CaM level increased. The causes of that staurosporine blocked 2 BS cells at G 1 phase but not effected on cell cycle progression in BGC-823 cells may be the results of staurosporine decreased the contents of CaM at G 1 phase and inhibited the phosphoralation of p107 in 2 BS cells.