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The Effect of Calmodulin Antagonists on Gibberellic Acid-induced Enzyme Secretion in Barley Aleurone Layers
Authors:OBATA  TORU; TANIGUCHI  HAJIME; MARUYAMA  YOSHIHARU
Institution:Division of Bio-function Research, Biomedical Research Laboratories, Jikei University School of Medicine Minato, 105 Tokyo, Japan
Laboratory of Biochemistry, Department of Agricultural Chemistry, University of Tokyo Bunkyo, 113
Abstract:Calmodulin activity was detected and assayed in barley aleuronecells. The effect of calmodulin antagonists on GA3-induced enzymesynthesis and secretion in barley aleurone layers was also investigated.These calmodulin antagonists (chlorpromazine, haloperidol) inhibitedonly GA2-induced {alpha}-amylase secretion. This inhibitory effectwas intensified after 6 h of GA3-incubation. This leads us tosuggest that some calmodulin-controlled mechanism is involvedin GA2-induced {alpha}-amylase secretion. Hordeum vulgare L., barley aleurone cells, gibberellic acid, {alpha}-amylase secretion, calmodulin, calmodulin antagonist
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