Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat |
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Authors: | Eujayl I Sorrells M E Baum M Wolters P Powell W |
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Institution: | (1) ICARDA, Germplasm Program, P.O. Box 5466, Aleppo, Syria, Present address: I. Eujayl, The S.R. Noble Foundation, 2510 Sam Noble PKWAY, Ardmore, OK, USA, US;(2) Department of Plant Breeding and Biometry, 520 Bradfield Hall, Cornell University, Ithaca NY 14853, USA, US;(3) DuPont Agriculture Biotechnology, DTP 200, 1 Innovation Way, Newark, DE 19714, USA, Present address: W. Powell, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK, US |
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Abstract: | Genetic variation present in 64 durum wheat accessions was investigated by using three sources of microsatellite (SSR) markers:
EST-derived SSRs (EST-SSRs) and two sources of SSRs isolated from total genomic DNA. Out of 245 SSR primer pairs screened,
22 EST-SSRs and 20 genomic-derived SSRs were polymorphic and used for genotyping. The EST-SSR primers produced high quality
markers, but had the lowest level of polymorphism (25%) compared to the other two sources of genomic SSR markers (53%). The
42 SSR markers detected 189 polymorphic alleles with an average number of 4.5 alleles per locus. The coefficient of similarity
ranged from 0.28 to 0.70 and the estimates of similarity varied when different sources of SSR markers were used to genotype
the accessions. This study showed that EST-derived SSR markers developed in bread wheat are polymorphic in durum wheat when
assaying loci of the A and B genomes. A minumum of ten EST-SSRs generated a very low probability of identity (0.36×10−12) indicating that these SSRs have a very high discriminatory power. EST-SSR markers directly sample variation in transcribed
regions of the genome, which may enhance their value in marker-assisted selection, comparative genetic analysis and for exploiting
wheat genetic resources by providing a more-direct estimate of functional diversity.
Received: 19 December 2000 / Accepted: 17 April 2001 |
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Keywords: | Durum wheat EST-SSR Genotyping Functional diversity |
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