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Preparative isolation and purification of chemical components from Aconitum coreanum by high-speed counter-current chromatography coupled with evaporative light scattering detection
Authors:Tang Qingfa  Yang Chunhua  Ye Wencai  Liu Jinghan  Zhao Shouxun
Affiliation:Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, People's Republic of China.
Abstract:Aconitum coreanum (Lèvl.) Rapaics (Guanbaifu in Chinese) is a widely used, centuries-old Chinese herb. A preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) method was employed for isolation and purification of alkaloids from the crude extract of Aconitum coreanum (Lèvl.) Rapaics using ethyl acetate-n-butanol-methanol-0.2 m HCl (7:2:2:7, v/v) as a two-phase solvent system. Six alkaloids, including GFO, GFQ, GFZ, hetisinone, hetisine and GFAA, were obtained in one-step separation. The purity of these compounds was 97.6, 93.8, 91.8, 91.9, 96.2 and 91.1%, respectively.
Keywords:Aconitum coreanum  counter‐current chromatography  diterpenoid alkaloids  plant materials
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