The cell wall receptor for bacteriophage Mu G(−) in Erwinia and Escherichia coli C |
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Authors: | Rodica Sandulache Peter Prehm Dominique Expert Ariane Toussaint Dietmar Kamp |
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Institution: | Max-Planck-Institut für Biochemie, 8033 Martinsried bei München F.R.G.;UniversitéLibre de Bruxelles, Facultédes Sciences, Rue des Chevaux 67, 1640 Rhode-St-Genèse, Belgium |
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Abstract: | Abstract Adsorption of bacteriophage Mu with its invertible DNA segment in the G(−) orientation requires a terminal glucose residue for binding to the core lipopolysaccharide (LPS) of Gram-negative bacteria. Analysis of a Mu-resistant mutant shows that the receptor for Mu G(−) in Erwinia B374 is a Glc-β1,6-Glc disaccharide. A spontaneously occurring host-range mutant, Mu G(−)h101, grows on Escherichia coli C. The loss of the terminal β1,3-linked glucose from the LPS of E. coli C leads to resistance to the phage Mu. These mutants are also resistant to phage P1 and D108 which have largely homologous G segments. This shows that Mu G(+) and G(−) phage particles differ with respect to their cell-wall receptors in the type of glycosidic linkage of a terminal glucose residue: α1, 2 for G(+) and β1,6 for G(−). |
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Keywords: | Invertible DNA segment host-range control lipopolysaccharide bacteriophage Mu P1 D108 |
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