Isolation and sequence determination of porcine class II DRB alleles amplified by PCR |
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Authors: | D I Våge I Olsaker F Lingaas Ø Lie |
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Institution: | Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, PO Box 8146, Dep., N-0033 Oslo, Norway |
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Abstract: | The first domain exon of a porcine DRB gene was amplified by the polymerase chain reaction (PCR), and the nucleotide sequence was determined. In a material consisting of 10 unrelated animals, five different alleles were identified, all probably belonging to a single locus designated DRB1. In addition, a non-expressed locus, designated DRBP, was coamplified with DRB1. This pseudogene, containing a single base deletion, also exhibited some variation, but at a very restricted level compared with DRB1. In pairwise comparisons of DRB1 alleles, the number of amino acid substitutions ranged between 6 and 21 out of 83 positions compared. |
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Keywords: | swine major histocompatibility complex DRB polymorphism |
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