An efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability |
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Authors: | Fan Junping Heng Jie Dai Shuyan Shaw Neil Zhou Bei Huang Bo He Zheng Wang Ya Jiang Taijiao Li Xuemei Liu Zhijie Wang Xianping Zhang Xuejun C |
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Institution: | a National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;b Key Laboratory for Cell Proliferation and Regulation Biology of the Ministry of Education, Institute of Cell Biology, Beijing Normal University,19 Xinjiekouwai Avenue, Beijing 100875, China |
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Abstract: | Membrane proteins account for about 30% of the genomes sequenced to date and play important roles in a variety of cellular functions. However, determining the three-dimensional structures of membrane proteins continues to pose a major challenge for structural biologists due to difficulties in recombinant expression and purification. We describe here a high throughput pipeline for Escherichia coli based membrane protein expression and purification. A ligation-independent cloning (LIC)-based vector encoding a C-terminal green fluorescence protein (GFP) tag was used for cloning in a high throughput mode. The GFP tag facilitated expression screening in E. coli through both cell culture fluorescence measurements and in-gel fluorescence imaging. Positive candidates from the GFP screening were subsequently sub-cloned into a LIC-based, GFP free vector for further expression and purification. The expressed, C-terminal His-tagged membrane proteins were purified via membrane enrichment and Ni-affinity chromatography. Thermofluor technique was applied to screen optimal buffers and detergents for the purified membrane proteins. This pipeline has been successfully tested for membrane proteins from E. coli and can be potentially expanded to other prokaryotes. |
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Keywords: | Transmembrane proteins High throughput Ligation-independent cloning Green fluorescence protein Thermofluor Protein expression |
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