Pilocarpine-Induced Seizures Produce Alterations on Choline Acetyltransferase and Acetylcholinesterase Activities and Deficit Memory in Rats |
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Authors: | Ítala Mônica de Sales Santos Chistiane Mendes Feitosa Rivelilson Mendes de Freitas |
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Institution: | 1.Laboratory of Physiology and Pharmacology,Federal University of Piaui,Piaui,Brazil;2.Laboratory of Chemistry,Federal University of Piaui,Piaui,Brazil |
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Abstract: | In the present study, we investigated the effect of seizures on rat performance in the Morris water maze task, as well as
on choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities in rat hippocampus. Wistar rats were treated
with 0.9% saline (i.p., control group) and pilocarpine (400 mg/kg, i.p., pilocarpine group). After the treatments all groups
were observed for 1 h. The changes on reference and working spatial memory caused by pilocarpine administration were observed
in seized rats. The ChAT and AChE activities were measured using spectrophotometric methods and the results compared to values
obtained from saline animals. Its activities were also determined after behavioral task. Results showed that seizures alter
reference memory when compared to saline-treated animals. In the working memory task, we observed a significant day’s effect
with significant differences between control and pilocarpine-induced seizures. In pilocarpine group, it was observed a significant
decreased in ChAT and AChE activities, when compared to control group. Our findings suggest that seizures caused cognitive
dysfunction and a decrease of ChAT and AChE activities that might be related, at least in part, to the neurological problems
presented by seizures induced by pilocarpine. |
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