Factors affecting lipid accumulation by <Emphasis Type="Italic">Nannochloropsis oculata</Emphasis> in a two-stage cultivation process |
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Authors: | Chia-Hung Su Liang-Jung Chien James Gomes Yu-Sheng Lin Yuan-Kun Yu Jhang-Song Liou Rong-Jhih Syu |
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Institution: | (1) Graduate School of Biochemical Engineering, Ming-Chi University of Technology, Taipei, 24301, Taiwan;(2) School of Biological Science, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India |
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Abstract: | Reserve lipids of microalgae are promising for biodiesel production. However, optimization of cultivation conditions for both
biomass yield and lipid production of microalgae is a contradictory problem because required conditions for both targets are
different. In this study, a two-stage cultivation strategy is proposed to enhance lipid production of the microalga Nannochloropsis oculata. Biomass growth and lipid production were carried out in two separate and non-interacting stages. In first-stage cultivation,
microalgae were cultivated in optimal conditions for cell growth. Then, microalgae were harvested and transferred into a growth-limited
environment, thus enhancing lipid production of microalgae. Here, optimization of the lipid production stage (second stage)
with respect to different levels of inoculum concentration, salinity of culture broth, and intensity of irradiance was performed.
The results show that irradiance exhibits a significant influence on lipid production. The highest lipid productivity of 0.324 g L−1 day−1 was obtained with an inoculum concentration of 2.3 g L−1, a salinity of 35 g L−1, and an irradiance of 500 μmol photons m−2 s−1. The final yield of lipid obtained from the two-stage process was 2.82-times higher than that from traditional single-stage
batch cultivation systems. |
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