Genome-wide identification and expression analysis of heat-responsive and novel microRNAs in Populus tomentosa |
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Authors: | Chen Lei Ren Yuanyuan Zhang Yiyun Xu Jichen Sun Fengshuo Zhang Zhiyi Wang Yanwei |
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Institution: | National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, People's Republic of China. |
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Abstract: | Plant microRNAs have a vital role in various abiotic stress responses by regulating gene expression. Heat stress is one of the most severe abiotic stresses, and affects plant growth and development, even leading to death. To identify heat-responsive miRNAs at the genome-wide level in Populus, Solexa sequencing was employed to sequence two libraries from Populus tomentosa, treated and untreated by heat stress. Sequence analysis identified 134 conserved miRNAs belonging to 30 miRNA families, and 16 novel miRNAs belonging to 14 families. Among these miRNAs, 52 miRNAs from 15 families were responsive to heat stress and most of them were down-regulated. qRT-PCR analysis confirmed that the conserved and novel miRNAs were expressed in P. tomentosa, and revealed similar expression trends to the Solexa sequencing results obtained under heat stress. One hundred and nine targets of the novel miRNAs were predicted. This study opens up a new avenue for understanding the regulatory mechanisms of miRNAs involvement in the heat stress response of trees. |
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Keywords: | miRNA microRNA miRNA* microRNA star sRNA small RNA HSP heat shock protein CSD1 Copper superoxide dismutase1 qRT-PCR quantitative real-time PCR ROS reactive oxygen species TH Populus tomentosa plantlet with heat treatment NH Populus tomentosa plantlet without heat treatment MFE The minimum free energy LRR Leucine-rich repeat APS ATP sulfurylase RISC RNA-induced silencing complex |
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