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A bioelectrochemical study of a suspension of Escherichia coli cells metabolizing glucose and lactose |
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| Authors: | G. Kh. Turishcheva I. A. Kazarinov O. V. Ignatov V. V. Ignatov |
| Affiliation: | (1) Chernyshevski Saratov State University, ul. Astrakhanskaya 83, Saratov, 410026, Russia;(2) Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410049, Russia |
| Abstract: | The metabolism of glucose and lactose in Escherichia coli K-12 cells has been studied using a bioelectrochemical (BEC) approach. The magnitude and the duration of the response of a BEC anode were found to be functions of the composition of nutrient media and the concentration of bacterial cells. The amount of electricity that is generated enzymatically during the metabolism of a particular substrate depends on the activity of the relevant enzymes. This suggests that the BEC approach can be used for evaluating the activity of particular enzyme systems. |
| Keywords: | metabolism cells mediator bioelectrochemical approach |
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