Antisense RNA decreases AP33 gene expression and cytoadherence by <Emphasis Type="Italic">T. vaginalis</Emphasis> |
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Authors: | V Mundodi AS Kucknoor JF Alderete |
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Institution: | (1) Department of Microbiology, University of Texas Health Science Center, 7703 Floyd Curl Drive, 78229-3900 San Antonio, TX, USA |
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Abstract: | Background Host parasitism by Trichomonas vaginalis is complex. Adherence to vaginal epithelial cells (VECs) is mediated by surface proteins. We showed before that antisense
down-regulation of expression of adhesin AP65 decreased amounts of protein, which lowered levels of T. vaginalis adherence to VECs. We now perform antisense down-regulation of expression of the ap33 gene to evaluate and confirm a role for AP33 in adherence by T. vaginalis. We also used an established transfection system for heterologous expression of AP33 in T. foetus as an additional confirmatory approach. |
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