全蝎抗癫痫发作敏感性的阿片肽机制

红藻氨酸（ＫａｉｎｉｃＡｃｉｄ，ＫＡ）癫痫模型，探讨全蝎抗癫痫发作敏感性长期增强的阿片肽机制。本实验选用ＳＤ大鼠，随机分为两组，分别给予生理盐水（ＮＳ）和全蝎粗提液灌胃１０天，１０天后两组均分别颈部皮下注射ＮＳ和惊厥剂量（１０ｍｇ／ｋｇ）的ＫＡ，再分别继续给予ＮＳ和全蝎粗提液灌胃１０天后，用阈下剂量（５ｍｇ／ｋｇ）的ＫＡ检测癫痫敏感性；用Ｆｏｓ免疫反应活性检测海马结构中神经元的兴奋性；用原位杂交技术检测海马脑啡肽原（ＰＥＮＫ）ｍＲＮＡ的动态变化过程。结果显示：实验对照组大鼠癫痫行为敏感性明显增强，脑内癫痫敏感性相关脑区海马齿状回颗粒细胞（ＤＧＣｓ）ｃ－Ｆｏｓ免疫反应阳性细胞数目明显增加，同时海马内具有致癫痫作用的脑啡肽原（ＰＥＮＫ）ｍＲＮＡ表达也明显增加；而实验组动物未见上述改变。本工作证实中药全蝎有明显降低海马神经元兴奋性及抗癫痫发作敏感性形成的作用，并提示这很可能与其抑制ＰＥＮＫｍＲＮＡ表达增加有关

THE OPIOID MECHANISM ON ANTI-SEIZURE SUSCEPTIBILITY BY SCORPION

Using Kainic acid(KA)seizure model to study the opioid mechanism of Scorpion antiseizure susceptibility longlasting enhancement The Sprague-Dawley rats received saline and Scorpion i g respectively for 10 days Then the rats were injected subcutaneously with convulsive dose of KA or the same volume of saline (NS) The rats which occurred seizure episodes were given NS and Scorpion i g for 10 days Then,using subconvulsive dose of KA to test the seizure susceptibility Fos immunohistochemistry and In situ were used to demonstrate the neuronal activity and the change of PENK mRNA in hippocampus respectively The seizure susceptibility in experimental control group rats increased significantly c-Fos and PENK mRNA expression increased in the dentate gyrus But the experimental group had no above changes The work verifies that Scorpion decreases neuronal activity and prevents the development of seizure susceptibility,which possibly relates to its inhibition on PENK mRNA expression enhancement